Efficient Agrobacterium Rhizogenes Mediated Hairy Root Culture System for Withanolide Production from Withania Somnifera (L.) Dunal
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منابع مشابه
Efficient Agrobacterium Rhizogenes Mediated Hairy Root Culture System for Withanolide Production from Withania Somnifera (L.) Dunal
Withania somnifera (L.) Dunal is an important medicinal plant with many pharmaceutical and therapeutic uses ranging from immunomodulation to anticancer property. Field cultivation of W. somnifera is time consuming and it not able to meet current global demands. For commercial production of withanolides, field grown plant materials have generally been used but the quality and uniformity in the l...
متن کاملImproved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment
An improved Agrobacterium rhizogenes-mediated genetic transformation of Withania somnifera (L.) Dunal was developed using the bacterial strain R1000 with leaf segment explants of in vitro raised plantlets. Out of the three strains used (R1000, MTCC 2364 and MTCC 532), the strain R1000 proved to be more efficient than others. Among the different conditions tested, the highest (93.3 %) transforma...
متن کاملIn vitro withanolide production by Withania somnifera L. cultures.
In vitro multiple shoots, root, callus and cell suspension cultures of Withania somnifera exhibited the potentiality to produce pharmacologically active withanolides. Multiple shoots cultures exhibited an increase in withanolide A accumulation compared to shoots of the mother plant. In vitro generated root cultures as well as callus and suspension cultures also produced withanolides albeit at l...
متن کاملCarbohydrate and elicitor enhanced withanolide (withaferin A and withanolide A) accumulation in hairy root cultures of Withania somnifera (L.).
Leaves of Withania somnifera contained more withaferin A and withanolide A than roots indicating that these compounds mainly accumulate in leaves. With an increase in age of the plant, withaferin A was enhanced with a corresponding decrease in withanolide A. Hairy root cultures were induced from leaf explants using Agrobacterium rhizogenes and the transgenic nature of hairy roots was confirmed ...
متن کاملA SIMPLE METHOD TO PURIFY WITHANOLIDE A FROM THE ROOTS OF Withania somnifera DUNAL
Withania somnifera, commonly known as Ashwagandha, is a valued herb in Ayurvedic medicine. Roots, leaves and preparations of the plant are traditionally used as tonic, hypnotic, sedative and diuretic. W.somnifera mainly contains withanolides which are specific to the Solanaceae family. Withanolides are biologically active secondary metabolites present in roots and leaves of W.somnifera. In the ...
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ژورنال
عنوان ژورنال: Journal of Applied Biotechnology & Bioengineering
سال: 2017
ISSN: 2572-8466
DOI: 10.15406/jabb.2017.03.00053